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Dermal exposure to the antimicrobial chemical triclosan augments allergic responses by inducing expression of thymic stromal lymphopoietin.

Marshall-NB; Lukomska-E; Long-CM; Nayak-AP; Meade-BJ; Anderson-SE

Toxicologist 2015 Mar; 144(1):143-144

https://www.toxicology.org/pubs/docs/Tox/2015Tox.pdf

20045895

The FDA is re-evaluating the safety and efficacy of triclosan, an antimicrobial chemical used in numerous products including soaps, dental and first aid supplies, toys, kitchenware, medical devices and clothing. The CDC reports that 75% of Americans have detectable levels of triclosan in their urine, and epidemiological studies have found a positive correlation between triclosan burden and diagnosis of allergic diseases. Mice exposed dermally to triclosan have also shown augmented allergic responses, however the mechanisms behind these effects are unknown. To further elucidate these mechanisms, the ear skin and draining lymph nodes at the site of dermal exposure were examined in BALB/c mice to identify early immunological changes induced by triclosan (0, 0.75 and 3% w/v). We discovered significant increases in thymic stromal lymphopoietin (TSLP) expression at the transcript and protein levels in the ear, but not in the lymph nodes or blood serum. In vivo administration of neutralizing anti-TSLP antibody impaired allergic responses augmented by dermal exposure to 3% triclosan during sensitization to ovalbumin. These effects include a significant decrease in skin pathology with a reduction in skin hyperplasia, redness and scabbing. There was also reduced cellularity of the skin draining lymph nodes, decreases in B cell frequencies, and reduced cytokine and GATA-3 transcription factor protein expression in Th2 CD4 T cells. These observations were further extended to human skin tissue cultures where we found that in vitro application of triclosan also induced TSLP expression. To our knowledge, this is the first report that triclosan can induce TSLP expression as a possible mechanism for augmenting allergic diseases.

Toxicology; Nanotechnology; Antibacterial-agents; Microorganisms; Allergies; Allergic-disorders; Allergic-reactions; Allergens; Laboratory-animals; Laboratory-testing; Skin-exposure; Immunological-tests; Lymph-nodes; Antigens; Proteins; Cellular-reactions; Immune-reaction; In-vivo-study; Antibody-response; Skin-disorders; Skin-irritants; In-vitro-study; Ears

3380-34-5

20150301

Abstract

2015

1

1096-6080

HELD

Healthcare and Social Assistance; Manufacturing

The Toxicologist. Society of Toxicology 54th Annual Meeting and ToxExpo, March 22-26, 2015, San Diego, California

WV; CA