NIOSHTIC-2 Search Results |
|
|
| Terms:
slaven |
13 - 13
of 35 Bibliographic entries |
|
Select check boxes to automatically save entries, or use 'save all' or 'save page' links above.
| 13 |
Discrimination of Aspergillus isolates at the species and strain level by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting |
| Authors |
Hettick-JM; Green-BJ; Buskirk-AD; Kashon-ML; Slaven-JE; Janotka-E; Blachere-FM; Schmechel-D; Beezhold-DH |
| Source |
Anal Biochem 2008 Sep; 380(2):276-281 |
| Link |
http://dx.doi.org/10.1016/j.ab.2008.05.051 |
| NIOSHTIC No. |
20034320 |
| Abstract | Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to generate highly reproducible mass spectral fingerprints for 12 species of fungi of the genus Aspergillus and 5 different strains of Aspergillus flavus. Prior to MALDI-TOF MS analysis, the fungi were subjected to three 1-min bead beating cycles in an acetonitrile/trifluoroacetic acid solvent. The mass spectra contain abundant peaks in the range of 5 to 20kDa and may be used to discriminate between species unambiguously. A discriminant analysis using all peaks from the MALDI-TOF MS data yielded error rates for classification of 0 and 18.75% for resubstitution and cross-validation methods, respectively. If a subset of 28 significant peaks is chosen, resubstitution and cross-validation error rates are 0%. Discriminant analysis of the MALDI-TOF MS data for 5 strains of A. flavus using all peaks yielded error rates for classification of 0 and 5% for resubstitution and cross-validation methods, respectively. These data indicate that MALDI-TOF MS data may be used for unambiguous identification of members of the genus Aspergillus at both the species and strain levels. | | Keywords | Fungi; Spectrographic-analysis; Spectroscopes; Statistical-analysis; Stains; Biomarkers | | Contact | Justin M. Hettick, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, 1095 Willowdale Road, Morgantown, WV 26505-2888 | | CODEN | ANBCA2 | | Publication Date | 20080915 | | Document Type | Journal Article | | Email Address | jhettick@cdc.gov | | Fiscal Year | 2008 | | NTIS Accession No. | | | NTIS Price | | | Issue of Publication | 2 | | ISSN | 0003-2697 | | NIOSH Division | HELD | | Priority Area | Services | | Source Name | Analytical Biochemistry | | State | WV |
|