Zou-Z-X; Wang-J; Wang-H; Li-Y-Q; Lin-Y
Talanta 2012 May; 94:58-64
A novel electrochemical biosensing device that integrates an immunochromatographic test strip and a screen-printed electrode (SPE) connected to a portable electrochemical analyzer was presented for rapid, sensitive, and quantitative detection of disease-related biomarker in human blood samples. The principle of the sensor is based on sandwich immunoreactions between a biomarker and a pair of its antibodies on the test strip, followed by highly sensitive square-wave voltammetry (SWV) detection. Horseradish peroxidase (HRP) was used as a signal reporter for electrochemical readout. Hepatitis B surface antigen (HBsAg) was employed as a model protein biomarker to demonstrate the analytical performance of the sensor in this study. Some critical parameters governing the performance of the sensor were investigated in detail. Under optimal conditions, this sensor was capable of detecting a minimum of 0.3 ng mL(-1) (S/N=3) HBsAg with a wide linear concentration range from 1 to 500 ng mL(-1). The sensor was further utilized to detect HBsAg spiked in human plasma with an average recovery of 91.3%. In comparison, a colorimetric immunochromatographic test strip assay (ITSA) was also conducted. The result shows that the SWV detection in the electrochemical sensor is much more sensitive for the quantitative determination of HBsAg than the colorimetric detection, indicating that such a sensor is a promising platform for rapid and sensitive point-of-care testing/screening of disease-related biomarkers in a large population.
Electrochemical-analysis; Electrochemical-properties; Antibody-response; Immune-reaction; Immune-system; Immunochemistry; Biochemical-indicators; Bioelectric-effects; Chromatographic-analysis; Biomarkers; Hepatitis; Antigens; Quantitative-analysis; Blood-samples; Humans; Testing-equipment; Peroxidases; Protein-biochemistry; Proteins; Analytical-chemistry; Analytical-instruments; Equipment-reliability; Detectors; Bioassays;
Author Keywords: Immunochromatographic test strip assay; Horseradish peroxidase; Hepatitis B; surface antigen; Disease-related biomarker
Yuehe Lin, Pacific Northwest National Laboratory, Richland, WA 99352, USA
Battelle Pacific Northwest Laboratories